The analysis of the monoclonal immune response to influenza virus. III. The relationship between stimulation of virus-primed precursor B cells by heterologous viruses and reactivity of secreted antibodies.
Identifieur interne : 002962 ( Main/Exploration ); précédent : 002961; suivant : 002963The analysis of the monoclonal immune response to influenza virus. III. The relationship between stimulation of virus-primed precursor B cells by heterologous viruses and reactivity of secreted antibodies.
Auteurs : W. GerhardSource :
- Journal of immunology (Baltimore, Md. : 1950) [ 0022-1767 ] ; 1978.
Descripteurs français
- KwdFr :
- MESH :
- biosynthèse : Anticorps antiviraux.
- immunologie : Cellules productrices d'anticorps, Lymphocytes B, Orthomyxoviridae.
- Animaux, Dosage radioimmunologique, Réactions croisées, Souris, Souris de lignée BALB C, Spécificité des anticorps.
English descriptors
- KwdEn :
- MESH :
- chemical , biosynthesis : Antibodies, Viral.
- immunology : Antibody-Producing Cells, B-Lymphocytes, Orthomyxoviridae.
- Animals, Antibody Specificity, Cross Reactions, Mice, Mice, Inbred BALB C, Radioimmunoassay.
Abstract
Individual splenic precursor B cells from BALB/c mice primed with influenza virus PR8[A/PR/8/34 (H0N1)] were stimulated in vitro in the splenic fragment culture system by homologous or various heterologous influenza viruses. The specificity of the stimulated precursor cells was determined by analysis of the antibodies secreted by the ensuing plasma cell clone in a radioimmunoassay (RIA). Viruses of the H2N2 and H3N2 subtypes were unable to stimulate hemagglutinin (HA)- or neuraminidase (NA)-committed precursor B cells but did efficiently stimulate chicken host component (ChHC)-committed precursors. Viruses of the H1N1 and H0N1 subtypes could stimulate precursors committed to any of the three viral surface components. Analysis of the fine specificity of HA-committed B cells showed that BEL(H0N1) and CAM(H1N1) stimulated almost exclusively precursors whose clonal antibody product reacted with the stimulating virus in the RIA. On the other hand, WSE and MEL (both H0N1) quite frequently were able to stimulate precursors whose clonal antibody product did not react with the stimulating virus in the RIA. These results suggest that the stimulatory interaction of viruses with the cell-bound immunoglobulin receptors is slightly less affinity dependent than the antigen-antibody interaction in the RIA.
PubMed: 305935
Affiliations:
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Le document en format XML
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III. The relationship between stimulation of virus-primed precursor B cells by heterologous viruses and reactivity of secreted antibodies.</title><author><name sortKey="Gerhard, W" sort="Gerhard, W" uniqKey="Gerhard W" first="W" last="Gerhard">W. Gerhard</name></author></analytic><series><title level="j">Journal of immunology (Baltimore, Md. : 1950)</title><idno type="ISSN">0022-1767</idno><imprint><date when="1978" type="published">1978</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibodies, Viral (biosynthesis)</term><term>Antibody Specificity</term><term>Antibody-Producing Cells (immunology)</term><term>B-Lymphocytes (immunology)</term><term>Cross Reactions</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Orthomyxoviridae (immunology)</term><term>Radioimmunoassay</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Anticorps antiviraux (biosynthèse)</term><term>Cellules productrices d'anticorps (immunologie)</term><term>Dosage radioimmunologique</term><term>Lymphocytes B (immunologie)</term><term>Orthomyxoviridae (immunologie)</term><term>Réactions croisées</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Spécificité des anticorps</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Antibodies, Viral</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Anticorps antiviraux</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Cellules productrices d'anticorps</term><term>Lymphocytes B</term><term>Orthomyxoviridae</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Antibody-Producing Cells</term><term>B-Lymphocytes</term><term>Orthomyxoviridae</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Antibody Specificity</term><term>Cross Reactions</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Radioimmunoassay</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Dosage radioimmunologique</term><term>Réactions croisées</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Spécificité des anticorps</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Individual splenic precursor B cells from BALB/c mice primed with influenza virus PR8[A/PR/8/34 (H0N1)] were stimulated in vitro in the splenic fragment culture system by homologous or various heterologous influenza viruses. The specificity of the stimulated precursor cells was determined by analysis of the antibodies secreted by the ensuing plasma cell clone in a radioimmunoassay (RIA). Viruses of the H2N2 and H3N2 subtypes were unable to stimulate hemagglutinin (HA)- or neuraminidase (NA)-committed precursor B cells but did efficiently stimulate chicken host component (ChHC)-committed precursors. Viruses of the H1N1 and H0N1 subtypes could stimulate precursors committed to any of the three viral surface components. Analysis of the fine specificity of HA-committed B cells showed that BEL(H0N1) and CAM(H1N1) stimulated almost exclusively precursors whose clonal antibody product reacted with the stimulating virus in the RIA. On the other hand, WSE and MEL (both H0N1) quite frequently were able to stimulate precursors whose clonal antibody product did not react with the stimulating virus in the RIA. These results suggest that the stimulatory interaction of viruses with the cell-bound immunoglobulin receptors is slightly less affinity dependent than the antigen-antibody interaction in the RIA.</div></front></TEI><affiliations><list></list><tree><noCountry><name sortKey="Gerhard, W" sort="Gerhard, W" uniqKey="Gerhard W" first="W" last="Gerhard">W. Gerhard</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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